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In db/db mice, the administration of BHB was shown to increase the levels of autophagy markers, including LC3 (Map1lc3a), p62 (Sqstm1), and beclin (Atg6), as well as enhance autophagic flux in the kidneys [9]

As a model for physiological conditions the cancer cells were cultivated and treated with a 21% O 2 atmosphere, and as a model for hypoxic conditions the cancer cells were cultivated and treated with a 1% O 2 atmosphere

The barrier integrity of the model was verified by permeability measurements for the transcellular biomarker EBA and small hydrophilic paracellular reference molecule fluorescein

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