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A 1:1 binding model was fitted to the thermogram using the Microcal PEAQ-ITC analysis software
The membrane filters were washed five times with a 200 l ice-cold wash buffer and transferred to a scintillation vial with 5Lml of Insta-Gel Plus scintillation cocktail (Perkin Elmer, 601339)
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(2018) 71:e127248
Although RBCs lack creatine kinase (CK), metabolites in the cytosol (ADP and glucose-6-phosphate) or enzymes in the cell membrane (adenylate kinase
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