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At 24 h after intravenous injection, the mice were sacrificed and tumor tissues and major organs (heart, liver, spleen, lung, kidney) were collected for ex vivo fluorescence imaging (Figure 5B)

For traceability, label all aliquots with compound name, concentration, reconstitution date, and operator initials

[25] The results were reproduced when NAD+ was replaced by giving several NAD+ precursor molecules, demonstrating that the positive effects are caused by NAD+ replacement

CD44-AF647 (Novus Biologicals, NB500-481AF488, clone MEM-263, lot P158343, FCy, 1:100, Hu)

For mouse serum collection, the animals were anaesthetized with isoflurane, and blood was collected via the retro-orbital venous sinus into BD microtainer tubes (BD, 365967)

mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation