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It also activates calpains that proteolytically cleave structural and functional proteins, including Na + /K + ATPase, NCE, SERCA, and the RyR, thereby perpetuating Ca 2+ influx and worsening myocardial injury [106, 108]

Always use aseptic techniques, protective equipment, and a clean, controlled laboratory environment

Cotransfection of the pMSCVLuciferase reporter vector containing the Gclc 3 UTR (WT or mutant) (1 g/well), a RenillaLuciferase (RenillaLuc) reporter construct (100 ng/well), and either mir18a mimic (50 nM) or control mimic (50 nM) was carried out

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So I was like, well, that's a conundrum

It deserves attention and care