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Total RNA was extracted from isolated islets and analyzed by rt-PCR to confirm the purity of the islets by the absence of amylase and CK19 at the transcriptional level

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min

They can make a custom dupe for you

Nustokite naudoti ir kreipkits gydytoj, jei atsiranda brimas

For established partners with regular purchasing schedules, we can accommodate customized ordering arrangements

The sensory signal is novel and merits further investigation in prospective studies