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S., Chiantera, V., Buzzaccarini, G., & Unfer, V
Key molecular mechanisms include ROS-mediated MAPK phosphorylation [375], triggering NLRP3 inflammasome assembly [376], oxidative modification of NF-B inhibitor proteins leading to their nuclear translocation [377], and induction of mitochondrial DNA mutations [378]
Glutathione is a powerful antioxidant found in every cell in the body
Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step
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RNA sequencing (RNA-seq) revealed a drastic induction of cystine transporter Slc7a11 (which encodes xCT) (~41-fold), Phgdh (~tenfold) and Psat1 (~ninefold) in mice fed HFCS and inulin compared to mice fed HFCS alone (Fig
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