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After centrifugation at 12,000 rpm for 20 min, the supernatant was collected for protein quantification via western blot analysis

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The mice were maintained on a 12-h light/dark cycle (lights on from 7:00 to 19:00) with access to food and water ad libitum

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It enriched with Glutathione Niacinamide and Centella Asiatica to increase skin elasticity while soothes irritated skin