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The two solutions were then combined and sterilized by filtration through a 0.22 m membrane
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Then, 50 L of 0.2 mol/L acetic acid containing d9-TMAO was added, followed by 150 L ice-cold trichloroacetic acid, and samples were incubated at 4 C for 10 min to precipitate proteins
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If you drink, keep within recommended limits, avoid binge drinking and discuss your individual situation with your healthcare team
