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This study aimed to compare the physiological roles of GIPR in the AP and hypothalamus by targeted regional KO of Gipr , using a floxed Gipr mouse model (Gipr fx/fx ) stereotactically injected with adeno-associated virus (AAV) encoding Cre-recombinase to generate mice with KO of Gipr in either the AP (Gipr AP-KO ) or hypothalamus (Gipr hypo-KO )

Neelam, Khatkar A, Sharma KK

Let's say your protocol calls for a 300mcg dose

That sequencing is not arbitrary

The signal is clear: high protein plus hard training during a deficit does not just preserve lean mass, it can build it