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By contrast, MII errors are associated with recombination occurring near the centromere of 21q (Lamb et al., 1997), which appears to depend on the increasing age of the oocyte (Oliver et al., 2012)

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For our high-throughput sequencing workflow, the following primers were used with Illumina Truseq adapter overhangs to amplify the substrate sequence from collected plasmids: ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNACGCTCACCGTAATGGTCAG (forward