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2.2 Laboratory-scale bioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702, Cytiva, Marlborough, MA) or in iCELLis Nano fixed-bed bioreactors (Pall Corporation, Port Washington, NY) with a 10 cm bed height with WMEM and 10% BCS controlled at a target temperature of 37C and pH of 7.2 as described previously ( 250 HT reactors (Sartorius AG, Gttingen, Germany) with 1 g/L Cytodex 1 microcarriers were operated for oncolytic virus production similar to a published method ( 250 HT bioreactors underwent an 80% medium exchange into WMEM without serum for a final concentration of 2% (v/v) BCS

(CPS)" "R12.4: A clear fluid diet should be encouraged 24 h before any procedure in patients taking GLP-1 agonists

Early induction of the Arabidopsis GSTF8 promoter by specific strains of the fungal pathogen Rhizoctonia solani

Stress pathways induced by these factors, including endoplasmic reticulum stress, oxidative stress, mitochondrial dysfunction, and autophagy, are all involved in the induction of -cell apoptosis, and these pathways may occur simultaneously at the same level or may interact to further exacerbate -cell death in the form of superimposed harmful stress

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