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The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody

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We were able to show that the circulating form of DPP4 (sDPP4), which lacks the cytoplasmic domain and the transmembrane region, is cleaved off the membrane of human adipocytes and smooth muscle cells in a process called shedding by the involvement of matrix metalloproteases (MMPs) ( Within the TMD, it could be shown that proline residues play an important role for the translocation of membrane-anchored proteins, such as DPP4

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309 Upon exposure to these treatments, a subset of cells enters a DTP state characterized by stem-like features, low proliferation, and heightened survival capacity

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