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After centrifugation at 60 g for 5 min at RT, the hepatocyte pellet was resuspended in attachment medium and mixed with an isotonic Percoll solution (1:1) followed by centrifugation at 230 g for 10 min at RT

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[25], they reported that animals pre-treated with AMPT showed lower locomotor activity, which was further confirmed by another research group [31]

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