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Reverse transcription and quantitative PCR (RT-qPCR) Total RNA was extracted using the RNeasy Mini Extraction kit (74104, Qiagen, Venlo, Netherlands), according to the manufacturers protocol

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Dopamine acts as a neurotransmitter, which passes signals between neurons and is essential in sending messages from the brain to direct muscle movement and coordination

A minimum 4-month rest period follows each cycle, with a maximum of 3 cycles per year

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Graves illness, thyroiditis, or toxic nodular goiter are all potential causes