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In contrast, mycelial growth of the Ltgpx3 mutants was significantly enhanced in calcofluor white (CFW)- and sorbitol-containing potato dextrose agar (PDA) plates compared to the WT (Fig

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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40