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HepG2 cells were purchased from ECACC and were cultured in Minimum Essential Medium supplemented with 10% FBS and 1% non-essential amino acids (Invitrogen)

This multifaceted communication has been named the gut-liver axis ( Indeed, alterations in the gut-liver axis characterized by increased portal and circulating levels of gut microbes/microbial components from compromised gut barrier and microbiota changes has emerged as central mediators in promoting ALD and MASLD progression ( The gut barrier Three main defense mechanisms make up the intestinal barrier function: (1) The immune barrier, which is made up of gut associated lymphoid tissue (GALT), effector and regulatory T cells, IgA-producing B cells, group 3 innate lymphoid cells, resident macrophages, and dendritic cells in the lamina propria

The researchers found that while elevated TMAO levels and enhanced susceptibility for platelet activation still occurred, the TMAO levels were attenuated by aspirin

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