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Oxidation of disulfide bonds of Pt GSTF8 Disulfide bonds between C13 of purified apo- Pt GSTF8 variants and GSH were formed by incubating 20 M Pt GSTF8 with 0 mM or 1 mM GSSG in the presence of 200 M GSH in 10 mM MOPSO, pH 6.45 for 2 h at 30 C, 800 rpm using a 3 mm shaking diameter

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Supplemental Tables 46 for specific model parameters) for the training of each tree, as a way to minimize overfitting

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