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2.6 Carnitine monooxygenase activity assays The standard L-carnitine depletion assay was performed as detailed under ( 2 , 150 mM NaCl containing 5 M purified CntA, 15 M purified CntB, and 2 mM L-carnitine at 27C
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All fermentation procedures were carried out inside a Ruskinn Concept 400 anaerobic workstation (Baker EMEA, Utrecht, Netherlands)
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