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Briefly, cells were lysed with NETN buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Nonidet P-40, 1 mM DTT) plus Xpert protease inhibitor cocktail solution (GeneDepot, Cat#P3100-001)
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Over time, that steady output can wear on nearly every system, from the heart and gut to sleep and mood, as the American Psychological Association outlines in its review of how stress affects the body
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