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In addition, pharmacological blockade of intracellular signals in both mouse and baboon cultures revealed that insulin requires different pathways from IGF-1 to exert a maximal inhibitory effect on GH expression/release [5]

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The cells were diluted to a certain density and then moved to polylysine (Sigma, P1399)-coated plates for culturing at 37 C in a 5% CO 2 incubator

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