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All the participants also took part in therapy designed to help reduce drinking
A systematic review & meta-analysis (2019) found that creatine supplementation did not significantly alter serum creatinine or plasma urea in healthy individuals (i.e., .) no evidence of renal damage A 2013 controlled study in resistance trained individuals on a high protein diet (1.2 g/kg/day), using gold-standard measured glomerular filtration rate (GFR by ^51Cr-EDTA clearance), found no change in kidney filtration over 12 weeks of creatine (loading + maintenance)
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The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody
In healthy individuals, the body produces sufficient quantities of these enzymes to manage normal dietary intake
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