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Participants who responded best to the supplement were often younger or had fewer metabolic complications
Cell viability assay Cell proliferation was detected via a CCK-8 assay (Biosharp, China) or a CellTiter-Glo assay (Promega) according to the manufacturers instructions
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Vitamin D also plays a modulatory role, increasing the expression of critical endogenous antioxidant enzymes, such as SOD1 (108)
This procedure was used to minimize potential interference from diet and medication
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