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We further evaluated the impact of metformin on OA progression in MIA-induced OA mice with Nrf2 deficiency

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However, it is unclear to what extent the in vitro observations correspond to metabolic and disease processes seen in vivo 12

This product has not been evaluated by the FDA and is supplied solely for in-vitro laboratory research by qualified professionals

Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 g ml 1 ampicillin and 25 g ml 1 kanamycin at 37 C to an OD 600 of 0.8 and induced with 0.1 mM isopropyl thio- -D-galactoside for 4 h

Vitamin and nutrition deficiencies are common