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The clinical potential of influencing Nrf2 signaling in degenerative and immunological disorders

ABSTRACT We aimed to study the effect of Cannabis sativa on brain oxidative stress and determine whether behavioral responses caused by cannabis could be reversed by standard antidepressant drugs

Hitomi H, Kiyomoto H, Nishiyama A

Readouts included protein expression by immunoblotting, free radical detection via flow cytometry quantification of %MitoSOX fluorescence (mitochondrial free radicals), ATP generation using fluorescence assay (Promega), and tricarboxylic acid (TCA) cycle intermediates by gas chromatography/mass spectrometry

While human clinical trials remain the gold standard, the breadth and consistency of animal research offers valuable insights into what users might expect

Redox Biology, 68:102967