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The reaction mixture was applied to a real-time PCR apparatus (Applied Biosystems 7500, USA)

Pharmacotherapy 32 , 10951111 (2012)

If it accumulates, in the presence of transition metal ions (such as Fe 2+ ), HO can undergo the Fenton reaction to generate the highly toxic OH [48]

Limitations of this study were the low dose of GABA and challenges with medication adherence as discussed previously