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The purified genomic DNA served as the template and was amplified by PCR (98 C for 30 sec, 25 (98 C for 10 s, 55 C for 20 s, 72 C for 20 s), 72 C for 5 min) with the forward primer S-D-Bact-0341-b-S-17 (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCW-GCAG-3) and reverse primer S-D-Bact-0785-a-A-21 (5-GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3) with Illumina adapters attached
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The rise of telemedicine clinics has also made it easier to access the medications, even for people who may not meet those criteria
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