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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

Cleavable linkers mean that the linker can be split during intracellular metabolism, which will produce metabolites containing the cytotoxic agent, which may have part of the linker

ACS Cent Sci 6(1):4153

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