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Hence, changes in the intracellular pH gradient resulted in decreased drug accumulation in tumor cells, thereby greatly reducing the efficacy of chemotherapeutic drugs and eventually leading to drug resistance

20 min for Huh7, 60 min for H1299), Tris-HCl neutralization (100 M, pH 8.5, 10 min) and Triton X-100 permeabilization (0.4%, 10 min)

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The Ukrainian and Russian protocols would require 1 or 2 50mg vials of epithalon per round of treatment, respectively

Krishna K, Paridhavi M, Patel J (2008) Review on nutritional, medicinal and pharmacological properties of papaya ( Carica papaya Linn.)