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After blocking with 5% BSA in TBST, slides were stained with rat anti-BrdU antibody (which detects CldU but not IdU, OBT0030, 1:2000, AbDSerotec) and mouse anti-BrdU antibody (which detects IdU but not CldU, 7580, 1:1000, BD Biosciences) at 4 C for overnight
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Cells are treated in triplicate with a serial dilution of IGF-1 LR3 for 4 hours
Franck, P., Moneret-Vautrin, D
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