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The peptide promoted tendon outgrowth from explants, enhanced tendon fibroblast migration in vitro, and increased cell survival under stress conditions
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Cell membranes were solubilized by 0.5% (w/v) LMNG (Anatrace, Maumee, USA) supplemented with 0.1% (w/v) CHS (Anatrace) with 5 M GLP-2 and Protease Inhibitor Cocktail (Topscience) at 4 C for 2 h, followed by centrifugation at 65,000 g for 30 min at 4 C