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To obtain additional restraints for structural model determination of the p53 TAD2 -FOXO4 FH complex, we determined paramagnetic relaxation enhancements (PREs) for a set of 7 complexes containing spin-labeled (SL) samples ( 15 N-labeled FOXO4 FH + S46C-SL p53 TAD2 , 15 N-labeled FOXO4 FH + Q52C-SL p53 TAD2 , 15 N-labeled p53 TAD2 + Q112C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + D140C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + N145C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S159C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S162C-SL FOXO4 FH )
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Peptide Amount % of Blend GHK-Cu (copper tripeptide) 50 mg 62.5% BPC-157 10 mg 12.5% TB-500 (thymosin beta-4 fragment) 10 mg 12.5% KPV (lysine-proline-valine) 10 mg 12.5% Total 80 mg 100% In a research context, each component maps to a distinct literature: GHK-Cu is the most-studied copper tripeptide, investigated for extracellular matrix remodeling and collagen-related signaling (Pickart & Margolina, 2018)
The tubular injury score was evaluated on the basis of the following morphologic changes, including necrotic tubules, loss of brush borders, tubule dilation, cast formation, tubular epithelial swelling, and vacuolar degeneration: 0, no morphologic deformities
Immune system issues
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