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Acknowledgements Support from the Translational Cancer Research Seed Grant, funded as 2010 Research Initiative Center by the State of South Dakota, and from NIH (5RO3CA133061-02) to GJB is gratefully acknowledged

Following mutagenesis, the plasmid template was digested using the Dpn I restriction enzyme to eliminate template plasmids and the plasmid was then transformed into competent E

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For experiments requiring multiple injections, rotating the injection sites helps maintain consistency and minimizes potential site-specific issues

Isolation of exosomes Mouse granulosa cells were acclimated in DMEM/F12 without exosomes for 24 h, and then subjected to different interventions