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The powder leaf sample (0.1 g) was homogenized with 1.5 mL of 1% Trichloroacetic acid (TCA) and the extract was centrifuged at 10,000 g at 4 C for 10 min

Effect of Alcohol Abuse and Glutathione Administration on the Circulating Levels of Glutathione and on Antipyrine Metabolism in Patients with Alcoholic Liver Cirrhosis. Scandinavian Journal of Clinical and Laboratory Investigation 56 (5): 44147

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During this time, their bodies may struggle to break down and use fats and sugars, particularly while fasting or during illnesses like gastroenteritis