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Samples were centrifuged for 10 min at 200 g at 4C, the supernatant was removed, and the cell pellet was resuspended in 2% BSA in PBS and the suspension was centrifuged for 10 min at 200 g at 4C

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People with certain medical conditions, abnormal lab results, cancer history, uncontrolled diabetes, pregnancy, or active endocrine disorders should be evaluated carefully before treatment