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The absorbance of the color developed was read at a wavelength of 412 nm after incubation at 25 C for 20 min

doi: 10.1046/j.1365-313X.1999.00403.x 146

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This is often the most noticeable during dose increases, and some studies have also reported that heart rate has also increased

Whether it plays out reliably in real skin is a separate question

However, other researchers have argued that controversial issues exist because GPNA also inhibits other glutamine transporters, such as SLC38A1, and V-9302 is effective even in SLC1A5 knockout models 206,207