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a , Dose-response curves showing relative cell number after 72 h of treatment of HCC-1806 cells with vincristine (0-5 nM) in the presence of the MRP1 inhibitor MK-571 (0-15 M) (n = 8 technical replicates from one experiment)
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The evidence base is largely preclinical (animal and cell) research on the individual components, supplemented by practitioner-derived protocols
Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis
Types Hemoglobin is made up of four protein chains: two alpha-globin chains, consisting of 4 genes (two from each parent), and two beta-globin chains, consisting of 2 genes (one from each parent)
Bodipy 665/676 (Thermo Fisher Scientific, B3932, 10 M, 45 min), LysoTracker Deep Red (Thermo Fisher Scientific, L12492, 100 nM, 45 min), DND-189 LysoSensor (Thermo Fisher Scientific, L7535, 100 nM, 1 h), Liperfluo (Dojindo, L248, 1 M, 1 h) and SQSS (in-house, 50 nM, 1 h) were added to live cells before fixation
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