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Sterile water lacks the bacteriostatic agent, meaning bacteria can begin to grow after the first use, contaminating the entire vial
Rheumatology (Oxford) (2006) 45(4):4214
A practical consequence of this coordination chemistry: any in vitro experiment that adds free GHK to cell culture medium containing serum (and therefore copper bound to albumin and other carrier proteins) is effectively delivering a mixture of free GHK and GHK-Cu, with the equilibrium dependent on copper availability
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GHK-Cu is not approved by the FDA for systemic therapeutic use (injection) as a drug
Specifically, increased temperature may promote reactive oxygen species (ROS) production and mitochondrial dysfunction, disrupt Sertoli cellgerm cell interactions, and induce apoptosis of spermatogenic cells
