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Building upon the earlier in vitro and in vivo findings, a study by Hung et al

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[5] Nevertheless, any signs of hypersensitivity (such as a diffuse rash, wheezing, or feeling of throat tightness) warrant stopping the injection and seeking prompt medical care

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The samples were diluted in RIPA, mixed with Laemmli buffer (Bio-Rad, Hercules, CA), and then denatured at 95C for 5 min