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The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described (Livak and Schmittgen, 2001)

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It has been reported that some domains of ACTL6A suggest that it is a member of the actin-related gene family, which is important in proteinprotein interactions 15

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To eliminate viral hepatitis as a public health threat, the US Department of Health and Human Services (HHS) released the Viral Hepatitis National Strategic Plan for the United States: A Roadmap to Elimination (20212025) in 2022

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Drugs 16:250

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Together, these examples show that with every use of chemical interventions off-targets for both the chemical and the solvent need to be ruled out for the proper interpretation of the results and mechanistic conclusions (Figure 3)

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