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RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)
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For A498, n of technical replicates are: Control = 6
& Chang, N.-C
Low glutathione levels are associated with chronic inflammatory conditions, including depression 4, type 2 diabetes 7 8, Alzheimers disease 9, cognitive decline 10, and autoimmune disorders 11
A 14 mg dose may be prescribed for further glycemic control
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