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The experiment involved several treatment groups across multiple animal models

Long-Term Risks of Vitamin B12 Overdose Persistent skin issues Nervous system irritation Blood pressure changes Increased cardiovascular stress excessive supplementation without medical oversight

It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile

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doi: 10.1515/CCLM.2007.362, 19

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