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Dominant negative G s (DNG s ) in pFastBac vector, and poly-his tagged G1 and G2 in a single pVL dual vector construct were used as previously described

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BPC 157 has beneficial effects on inflammation, hemorrhage, and edema after traumatic brain injury [33], various severe encephalopathies (which follow gastrointestinal and/or liver lesions), NSAID overdose [34,35,36,37], or insulin overdose seizures [38] and on severe muscle weakness after exposure to the specific neurotoxin cuprizone in a rat multiple sclerosis model [39] or magnesium overdose [40]