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Changes in mitochondrial morphology and bioenergetics in human lymphoblastoid cells with four novel OPA1 mutations

Avoid repeated freeze thaw cycles

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To obtain plasma, typically whole blood is collected into tubes pre-coated with an anticoagulantheparin and EDTA being the most commonand then transported to the laboratory on wet ice where the whole blood is centrifuged to fraction the sample into its component parts (red blood cells, white blood cells, and plasma)