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Routes of Administration Subcutaneous injection (standard): Protocols describe injecting into the abdominal area (2+ inches from navel), thigh, or upper arm using a 29-31 gauge insulin syringe, rotating injection sites

That does not invalidate DSIPs use as a subcutaneously injected peptide, but it changes the degree of certainty you should expect with the results you get

Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer