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Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort

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Furthermore, because AST is a carotenoid with lipophilic properties, there is concern about its potential to accumulate in tissues with prolonged use

Mix smaller batches more frequently Strategy: Instead of reconstituting entire 10mg vial to last 8 weeks, reconstitute 5mg to last 4 weeks

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Longer exposure studies are needed to analyze changes in testicular histology and controlled clinical studies will help to clarify the impact of Modified Risk Tobacco Products on humans