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All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction
In contrast, long acting GLP-1 receptor agonists have relatively little effect on the rate of gastric emptying due to the development of tachyphylaxis resulting from continuous activation of the GLP-1 receptors in the GI tract (Nauck et al, 2011
It's assuming the peptide is stable at ambient temperature during multi-day protocols
These amino acids can then enter cells and be used to make glutathione again
