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Aliquoting is considered standard practice to preserve consistency across experiments

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Critical revision of the manuscript for important intellectual content: all authors

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As a result of overexpression GGCT, not only did the level of glutamate m + 5 decrease, but also the levels of downstream metabolites such as -KG m + 5, succinate m + 4, fumarate m + 4 and malate m + 4 in the TCA cycle were affected (Fig