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Chondrocytes were transfected with GLP-1R siRNA (GeneChem, Shanghai, China) using lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA) according to the instruction

TGF1, via its downstream Ras homolog family member A (RhoA)/rho-associated coiled coil-containing protein kinase (ROCK) signaling pathway, stimulates the abnormal distribution of F-actin and G-actin and stress fiber accumulation, creating a barrier for GLP-1 secretion

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