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The metabolic programs identified in this study were inferred from transcriptomic data
This is the same principle behind why recombinant protein biologics (monoclonal antibodies, insulin analogues) are never shaken: air-water interfacial agitation unfolds polypeptide chains that then reassemble into non-native oligomers or fibrils
Subsequently, this fixed tissue was embedded in the Tissue-Tek OCT compound (Sakura Finetek, Tokyo, Japan), frozen, and stored at 80C for cryosectioning
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We are especially grateful to Rutherford Latham and Alfonso Arango (Jumping riders) for excellent collaborations and discussions
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