Application of molecular techniques for diagnostic purposes could be implemented with an established G6PD mutation database (77, 78)
Additionally to the glycine biosensor, we have also designed and built constructs constitutively expressing the GcvA transcription factor, GcvR transcription factor and pepD using the BBa_J23100 promoter found on the iGEM registry of Basic Biological Parts
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F., Gaddi, A., Mugellini, A., Ciccarelli, L., and Fogari, R
Narita, S
Following dilutions of antihuman primary antibodies in PBS were applied overnight at 4 C: Nestin (Millipore, MAB5326, 1:200), SOX2 (Abcam, ab97959, 1:200), Doublecortin (Cell Signaling, 4604S, 1:200), MAP2 (Millipore, MAB3418, 1:200), PSA-NCAM (Millipore, MAB5324, 1:200), PSD95 (Abcam, ab18258, 1:200) Tubulin, beta 3 (Millipore, MAB1637, 1:200)